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Image Search Results
Journal: Journal of Cerebral Blood Flow & Metabolism
Article Title: ABC transporter function and regulation at the blood-spinal cord barrier
doi: 10.1038/jcbfm.2012.47
Figure Lengend Snippet: ATP binding cassette (ABC) transporter function and expression in spinal cord and brain capillaries from control and TCDD-dosed rats (5 μg/kg intraperitoneally in corn oil; capillaries isolated 48 hours after dosing). (A) P-glycoprotein transport activity. (B) P-glycoprotein (P-GP) expression. (C) Mrp2 and Bcrp expression. (D) Electrophoretic mobility shift assay (EMSA) shows nuclear translocation of arylhydrocarbon receptor (AhR) in spinal cord capillaries from TCDD-dosed rats. Each bar represents the mean value for nine capillaries from a single preparation (pooled tissue from 10 rats); variability is shown as standard error bars. Statistical comparisons: ***significantly different from control, P<0.001. TCDD, 3,7,8-tetrachlorodibenzo-p-dioxin; DRE, dioxin response element; Mrp2, multidrug resistance protein 2; Brcp, breast cancer-related protein.
Article Snippet: The EMSA (Electrophoretic Mobility Shift Assay) samples were prepared using
Techniques: Binding Assay, Expressing, Isolation, Activity Assay, Electrophoretic Mobility Shift Assay, Translocation Assay